Optimization of Extraction of Cannabinoids and Flavonoids Substances from Influence <i>Cannabis sp</i>. and their Biological Activities before and after Decarboxylation

Inflorescence of eight different varieties Cannabis sp. (3 hemp and 5 medical cannabis varieties) were subjected to extraction with n-hexane or ethanol as representatives non-polar polar solvents. Crude extracts either used directly after decarboxylation acids for measurement selected biological activities - cytotoxicity against carcinogenic line B16 (musculus skin melanoma cells), Human Dermal Fibroblast (HDF) a control non-carcinogenic line, antimicrobial activity Candida albicans (DBM, 2186), Staphylococcus aureus (ATCC, 25923) Salmonella enterica (CCM, 4420) antioxidant using the ORAC (Oxygen Radical Absorbance Capacity) assay. 32 samples profile cannabinoids measured by HLPC thus obtained evaluated. The content total flavonoids that could exert synergic action on bioactivity was spectrophotometric method. hexane showed higher activity, led an increase in human fibroblast most studied samples, decrease effects decarboxylated extracts. findings suggest therapeutic potential treatment cancer, wounds, surgical wound healing.